Label-Free Molecular Interaction Analysis System

Notice of Intended Procurement NAME OF PROCURING ENTITY The Governing Council of the University of Toronto (the “University”) CONTACT PERSON AND CONTRACT PERSON COORDINATES Renee Cheng 150 College Street, 3rd Floor, Room 350, Toronto ON M5S 1A2 reneeke.cheng@utoronto.ca PROCUREMENT DOCUMENTS The procurement documents are available at www.merx.com. Note that obtaining access to the procurement documents will require prospective suppliers to register and pay a registration fee. Pricing and Payment Terms are available on the MERX website. DESCRIPTION OF PROCUREMENT The Pardee Lab in the Leslie Dan Faculty of Pharmacy (LDFP) at the University of Toronto (UofT), conducts research at the St. George campus at the interface of cell-free synthetic biology, biomolecular engineering, and molecular diagnostics and therapeutics. The Label-Free Molecular Interaction Analysis System (the “System”) described in this RFP is to be purchased under the CFI grant, as described in section 1.7(1) of this RFP and should meet the applicable CFI eligibility and requirements. The Pardee Lab seeks a biophysical molecular interaction analysis instrument to support quantitative characterization of biomolecular binding by measuring real-time binding kinetics (on-rate, off-rate) and affinity (KD), analyte quantitation, and competitive inhibition (Ki) measurements, across the range of biomolecules produced and engineered. The System may be shared in collaboration with other LDFP labs performing therapeutic discovery workflows for peptides, peptidomimetics, and small molecules using the System to validate and kinetically characterize candidate binders. The System will perform real-time, label-free measurement of molecular interactions to determine binding affinity, binding kinetics, analyte concentration, and competitive binding relationships among proteins, antibodies, peptides, and nucleic acids (DNA/RNA), in both purified and crude sample formats (including cell-free expression lysates, cell cult